We have already mentioned the complex process leading to the appearance of tumors in the human body. The question justly arises as to where and how deuterium depletion can have a positive influence on the outcome of this complex process. It is also clear that the appearance of tumors can be attributed to two main causes: one being an increase in the number of genetic defects, the other the increase of genetically defective (tumorous) cells. The former results in the greater probability of the tumorous nature of a given cell, the latter leads to the fact that with an increase among cells of a tumorous nature, there will probably be one or more tumor cells which, due to newer genetic defects will be able to evade obstacles preventing tumor growth, rendering cell proliferation utterly uncontrollable.
In connection with the "anti-cancer" effect of deuterium depletion we do not claim that by consuming Dd-water the number of mutations is decreased, although there are numerous arguments for this as well. (It has been experimentally proven by that in a medium with a higher than natural D-concentration, the number of mutations increases, so there is a good reason to presume that the presence of deuterium can be considered to be a mutation factor, thus, low D-concentration could as well decrease the frequency of the incidence of mutations.)
Of greater importance is to analyze the effect of deuterium depletion on tumor cells. Based on our observations so far, we can state that with the consumption of Dd-water the lowering of D-concentration may inhibit tumor growth and consequently may lead to partial or complete tumor regression.
This effect of Dd-water is reinforced by several data:
1) Cell proliferation was inhibited in Dd-water treated in vitro culture and, within our experimental system, deuterium depletion induced apoptosis.
2) In the case of tumor transplants in mice, cats and dogs with spontaneous tumors as well as in cancer patients, the size of tumors stagnated or decreased as a result of Dd-water consumption. Experiments with mice also verified that deuterium depletion inhibited the proliferation of tumor cells and resulted in their destruction inside the tumor: the process resulted in a decrease of the tumor volume.
3) One indirect proof of the presence of certain tumors is the appearance of specific proteins (tumor markers) in the blood. After the consumption of Dd-water (in some cases after a temporary increase), the value of tumor markers may decrease. This decrease is connected to the decrease of tumor volume, which also reinforces the anti-cancer effect of deuterium depletion.
4) Several examples have shown that in total remission (when in treated cancer patients there is no proof of the presence of tumor cells), in some cases there was no relapse for years when the patient had been drinking Dd-water, but when the consumption was stopped, the tumor appeared again within several months. This reveals that Dd-water helped these patients maintain an internal balance and inhibited tumor growth.
5) In developed countries numerous investigations were conducted for over 10 years – with the participation of several thousand patients – to prove the connection between nutrition or drug usage, and the more or less frequent occurrence of certain diseases. One of these experiments has convincingly shown that among patients who regularly take NSAIDs (non-steroid anti-inflammatory drugs such as e.g. aspirin) certain cancers (lung, colon) occur less frequently. Basic research has also proved that this effect is due to the fact that some drugs inhibit the gene COX-2 that has a role in prostaglandin synthesis. Our research has likewise shown that deuterium depletion inhibits COX-2 activity.
6) During recent months we have proved successfully that deuterium depletion inhibits the activity of those genes whose role in cancer growth has been known to scientists for years.
Our basic research results are in harmony with the work of researchers abroad and prove – both macroscopically and on a molecular level – the anti-tumor effect of deuterium depletion. We suppose that the presence of deuterium is vital for cell proliferation and that cell division is triggered by the change of the D/H ratio (the ratio of D temporarily increases in contrast to that of H). When the patient consumes water of a normal D-content, tumor cells have no problem ensuring the D/H ratio needed for division. However, when we decrease the D-concentration of the body through deuterium depletion, either the conditions for cell division do not exist, or the tumor cell can only achieve this ratio considerably later. We thus deprive tumor cells of a most important factor: the possibility to ensure conditions for cell division.
As shown earlier, cells are able to adapt to several external effects. Over time there will always be some which are able to achieve – through a genetic change – an advantage over other cells and, by outgrowing their surroundings, to overcome the obstacle. These facts make deuterium depletion an effective tool in tumor therapy but they also set the limits of application. Deuterium depletion might be a successful means of destroying tumor cells because, in the adaptation process, healthy cells have an advantage over defective ones. This means that the unit decrease of D-concentration induces a stress in both healthy and tumor cells but healthy ones quickly adapt to the lower D-concentration, whereas tumor cells are unable to do so or most adapt much more slowly. If, in the meantime, D-concentration continuously decreases, the tumor cell is again subject to a newer stress, even though it was not able to manage the previous one. With the appropriate dosage of Dd-water, this process may result in the destruction of all cells constituting the tumor.
The constraints of the treatment are that the tumor, even if slowly, nevertheless is able to adapt to the new situation. The bigger the tumor and the more tumor cells the patient has, the easier. Unfortunately, there is a chance that after a certain time cells able to adjust to D-depletion will appear, resulting in further tumor growth. One of the directions of our drug development is to further enhance the efficacy of D-depletion by adding other materials. Another option is to administer Dd-water at the earliest possible stage together with other treatments even if the patient, due to surgical or other treatments, is tumor-free because in such cases the number of possible tumor cells is minimal, giving us the greatest chance to destroy them.